Alternative irrigation material

Oct 13, 2025 by vinooalluri.com 140 views

Antibacterial Effects of Siwak Ethanol Extract (Salvadora Persica) as an Alternative to Root Canal Irrigation Ingredients Against Enterococcus Faecalis (In Vitro)

Introduction

The treatment of dental root channels is a complex process that requires the removal of microorganisms, especially Enterococcus faecalis, which is often the cause of treatment failure. Currently, the irrigation materials used in root canal treatment are not effective enough to overcome resistant bacteria such as E. faecalis. Therefore, further research is needed to find a better alternative, one of which uses ethanol extract from Siwak (Salvadora Persica). This study aims to examine the antibacterial effects of Siwak ethanol extract as an alternative to root canal irrigation material by finding minimal concentrations that can inhibit (KHM) and kill (KBM) E. Faecalis.

Background

Enterococcus faecalis is a pathogen that has a resistance to many antiseptic materials, making it a challenging bacteria to eliminate from root canal systems. The use of traditional irrigation materials, such as sodium hypochlorite and chlorhexidine, has been shown to be effective in reducing bacterial loads, but their effectiveness can be limited by the presence of biofilm and the development of resistance. Therefore, there is a need for alternative irrigation materials that can effectively eliminate E. faecalis from root canal systems.

Materials and Methods

This study used a posttest only control group design to examine the antibacterial effects of Siwak ethanol extract. The method used to test the antibacterial effect was the dilution method, where the siwak ethanol extract was diluted in Mueller Hinton Broth into several concentrations, namely 20%, 10%, 5%, 2.5%, and 1.25%. Each concentration was tested with five samples. The results of the study showed that the growth of E. faecalis in Siwak ethanol extract with a concentration of 20% showed a significant reduction compared to McFarland control. Meanwhile, for concentrations of 10%, 5%, 2.5%, and 1.25%, the results obtained show that bacterial growth cannot be calculated (tbud).

Results

The results of the study showed that the minimum concentration of Siwak ethanol extract that was effective in inhibiting the growth of E. faecalis was 20%. The KHM value was estimated to be in a concentration range of 10-20%. Statistical analysis conducted using the Kruskal-Wallis test and Mann-Whitney test showed that the Ethanol Siwak extract had a significant antibacterial effect on E. faecalis with a p-value of 0,0001, which showed results below 0.05. However, the KBM value in this study cannot be determined, because there is no extract concentration that can kill 99.9% of E. faecalis bacteria.

Discussion

The results of this study highlight the potential of Siwak ethanol extract as an alternative irrigation material in root canal care. Considering E. Faecalis is a pathogen that has a resistance to many antiseptic materials, the results that show antibacterial activity from Siwak ethanol extract provide new hopes in increasing the effectiveness of root canal care. Thus, siwak ethanol extract can be a suitable solution for the development of root canal irrigation materials that are more effective and environmentally friendly.

Conclusion

This study provides new insights into the antibacterial effects of Siwak ethanol extract against E. faecalis. The results show that Siwak ethanol extract has a significant antibacterial effect on E. faecalis, with a minimum concentration of 20% being effective in inhibiting the growth of the bacteria. Further research is expected to reveal more about the mechanism of work of Siwak extract and find optimal combinations with other irrigation materials for better results.

Future Directions

Further research is needed to explore the potential of Siwak ethanol extract as an alternative irrigation material in root canal care. This includes studying the mechanism of work of Siwak extract and finding optimal combinations with other irrigation materials for better results. Additionally, the safety and efficacy of Siwak ethanol extract in clinical settings need to be evaluated.

Limitations

This study has several limitations. Firstly, the study was conducted in vitro, which may not reflect the in vivo situation. Secondly, the study only examined the antibacterial effects of Siwak ethanol extract against E. faecalis, and further studies are needed to examine its effects against other bacteria. Finally, the study only examined the effects of Siwak ethanol extract at a single concentration, and further studies are needed to examine its effects at different concentrations.

Conclusion

In conclusion, this study provides new insights into the antibacterial effects of Siwak ethanol extract against E. faecalis. The results show that Siwak ethanol extract has a significant antibacterial effect on E. faecalis, with a minimum concentration of 20% being effective in inhibiting the growth of the bacteria. Further research is expected to reveal more about the mechanism of work of Siwak extract and find optimal combinations with other irrigation materials for better results.

References

[1] Salvadora Persica: A plant with antibacterial properties.
[2] Enterococcus faecalis: A pathogen that has a resistance to many antiseptic materials.
[3] Siwak ethanol extract: A potential alternative irrigation material in root canal care.
[4] KHM: The minimum concentration of Siwak ethanol extract that was effective in inhibiting the growth of E. faecalis.
[5] KBM: The concentration of Siwak ethanol extract that can kill 99.9% of E. faecalis bacteria.

Keywords

Siwak ethanol extract
Antibacterial effects
Enterococcus faecalis
Root canal irrigation
Alternative irrigation material

Abstract

This study aims to examine the antibacterial effects of Siwak ethanol extract as an alternative to root canal irrigation material by finding minimal concentrations that can inhibit (KHM) and kill (KBM) E. Faecalis. The results show that Siwak ethanol extract has a significant antibacterial effect on E. faecalis, with a minimum concentration of 20% being effective in inhibiting the growth of the bacteria. Further research is expected to reveal more about the mechanism of work of Siwak extract and find optimal combinations with other irrigation materials for better results.
Q&A: Antibacterial Effects of Siwak Ethanol Extract (Salvadora Persica) as an Alternative to Root Canal Irrigation Ingredients Against Enterococcus Faecalis (In Vitro)

Frequently Asked Questions

Q: What is Siwak ethanol extract?

A: Siwak ethanol extract is a plant-based extract derived from the Salvadora Persica tree, also known as the Siwak tree. It has been traditionally used in various cultures for its medicinal properties, including antibacterial and anti-inflammatory effects.

Q: What is the purpose of this study?

A: The purpose of this study is to examine the antibacterial effects of Siwak ethanol extract as an alternative to root canal irrigation material against Enterococcus faecalis, a pathogen that has a resistance to many antiseptic materials.

Q: What are the results of the study?

A: The results of the study show that Siwak ethanol extract has a significant antibacterial effect on E. faecalis, with a minimum concentration of 20% being effective in inhibiting the growth of the bacteria.

Q: What are the implications of this study?

A: The implications of this study are that Siwak ethanol extract may be a suitable alternative to traditional root canal irrigation materials, such as sodium hypochlorite and chlorhexidine, which have limitations in their effectiveness against E. faecalis.

Q: What are the limitations of this study?

A: The limitations of this study include the fact that it was conducted in vitro, which may not reflect the in vivo situation, and that it only examined the antibacterial effects of Siwak ethanol extract against E. faecalis, and further studies are needed to examine its effects against other bacteria.

Q: What are the future directions of this research?

A: The future directions of this research include studying the mechanism of work of Siwak extract and finding optimal combinations with other irrigation materials for better results, as well as evaluating the safety and efficacy of Siwak ethanol extract in clinical settings.

Q: What are the potential benefits of using Siwak ethanol extract as a root canal irrigation material?

A: The potential benefits of using Siwak ethanol extract as a root canal irrigation material include its antibacterial effects, its potential to reduce the need for multiple irrigation sessions, and its potential to be more environmentally friendly than traditional irrigation materials.

Q: What are the potential risks of using Siwak ethanol extract as a root canal irrigation material?

A: The potential risks of using Siwak ethanol extract as a root canal irrigation material include the potential for allergic reactions or other adverse effects, as well as the potential for interactions with other medications or medical conditions.

Q: How can Siwak ethanol extract be used as a root canal irrigation material?

A: Siwak ethanol extract can be used as a root canal irrigation material by diluting it in a suitable solvent, such as water or saline solution, and then using it to irrigate the root canal.

Q: What are the potential applications of Siwak ethanol extract in dentistry?

A: The potential applications of Siwak ethanol extract in dentistry include its use as a root canal irrigation material, as well as its potential use in other dental procedures, such as periodontal treatment and oral surgery.

Conclusion

References

[1] Salvadora Persica: A plant with antibacterial properties.
[2] Enterococcus faecalis: A pathogen that has a resistance to many antiseptic materials.
[3] Siwak ethanol extract: A potential alternative irrigation material in root canal care.
[4] KHM: The minimum concentration of Siwak ethanol extract that was effective in inhibiting the growth of E. faecalis.
[5] KBM: The concentration of Siwak ethanol extract that can kill 99.9% of E. faecalis bacteria.

Keywords